WikiDoc Resources for Alkaline lysis Articles Most recent articles on Alkaline lysis Most cited articles on Alkaline lysis Review articles on Alkaline lysis Articles on Alkaline lysis in N Eng J Med, Lancet, BMJ Media Powerpoint slides on Alkaline lysis Images of Alkaline lysis Photos of Alkaline lysis Podcasts & MP3s on Alkaline lysis Videos on Alkaline lysis Evidence Based Medicine Cochrane Collaboration on Alkaline lysis Bandolier on Alkaline lysis TRIP on Alkaline lysis Clinical Trials Ongoing Trials on Alkaline lysis at Clinical Trials.gov Trial results on Alkaline lysis Clinical Trials on Alkaline lysis at Google Guidelines / Policies / Govt US National Guidelines Clearinghouse on Alkaline lysis NICE Guidance on Alkaline lysis NHS PRODIGY Guidance FDA on Alkaline lysis CDC on Alkaline lysis Books Books on Alkaline lysis News Alkaline lysis in the news Be alerted to news on Alkaline lysis News trends on Alkaline lysis Commentary Blogs on Alkaline lysis Definitions Definitions of Alkaline lysis Patient Resources / Community Patient resources on Alkaline lysis Discussion groups on Alkaline lysis Patient Handouts on Alkaline lysis Directions to Hospitals Treating Alkaline lysis Risk calculators and risk factors for Alkaline lysis Healthcare Provider Resources Symptoms of Alkaline lysis Causes & Risk Factors for Alkaline lysis Diagnostic studies for Alkaline lysis Treatment of Alkaline lysis Continuing Medical Education (CME) CME Programs on Alkaline lysis International Alkaline lysis en Espanol Alkaline lysis en Francais Business Alkaline lysis in the Marketplace Patents on Alkaline lysis Experimental / Informatics List of terms related to Alkaline lysis Alkaline lysis is a method used in molecular biology to break cells open to isolate plasmid DNA or other cell components such as proteins. Bacteria containing the plasmid of interest is first grown, then lysed with a strong alkaline buffer consisting of a detergent sodium dodecyl sulfate (SDS) and a strong base sodium hydroxide. The detergent breaks the membrane's phospholipid bilayer and the alkali denatures proteins involved in maintaining the structure of the cell membrane. Through a series of steps involving agitation, precipitation, centrifugation, and the removal of supernatant, cellular debris is removed and the plasmid is isolated and purified. The protocol may vary slightly from lab to lab. Also, alkaline lysis is sometimes used to extract plant genetic material. The plant cells are subjected to a strongly alkaline solution containing a detergent (usually a zwitterionic or nonionic detergent such as Tween 20), and the mixture is incubated at high temperature. This method is not used as often due to the sodium hydroxide's tendency to damage genetic material, reducing DNA fragment size. ## References[edit | edit source] * Alkaline Lysis at Mama Ji's Molecular Kitchen * A rapid alkaline extraction procedure for screening recombinant plasmid DNA by H. C. Birnboim and J. Doly Template:WH Template:WS